DIFFERENTIAL INDIRECT FLUORESCENCE DETECTION FOR THE REVERSED-PHASE MICROCOLUMN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

KAWAZUMI, H ^{[1
]}

NISHIMURA, H ^{[1
]}

OGAWA, T ^{[1
]}

机构：

[1] KYUSHU UNIV,DEPT MOLEC SCI & TECHNOL,KASUGA,FUKUOKA 816,JAPAN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 13期

关键词：

D O I：

10.1080/10826079208016174

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential indirect fluorescence detection was applied for reversed-phase microcolumn high-performance liquid chromatography of aliphatic alcohols. The differential measurement with single capillary cell and double beams improved dynamic reserve and detectability. The indirect detection system suitable for a visible semiconductor laser was developed with a deep-red dye, 3, 3'-dimethyloxatricarbocyanine chloride. A mass detection limit was in the sub-mu-g (nmol) range.

引用

页码：2233 / 2245

页数：13

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