PURIFICATION AND CHARACTERIZATION OF A DIPTERICIN HOMOLOG FROM SARCOPHAGA-PEREGRINA (FLESH FLY)

被引：40

作者：

ISHIKAWA, M ^{[1
]}

KUBO, T ^{[1
]}

NATORI, S ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL JOURNAL | 1992年 / 287卷

关键词：

D O I：

10.1042/bj2870573

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein with a molecular mass of 8 kDa was found to be synthesized specifically when the fat-body from injured Sarcophaga peregrina larvae was cultured in vitro. This protein was purified from the haemolymph of the injured larvae to near-homogeneity. Partial amino acid sequencing revealed that this protein is a diptericin homologue. It showed bactericidal activity on growing, but not resting Escherichia coli cells. E. coli cells become elongated on treatment with this protein.

引用

页码：573 / 578

页数：6

共 30 条

[1] INHIBITORY EFFECT OF SARCOTOXIN-IIA, AN ANTIBACTERIAL PROTEIN OF SARCOPHAGA-PEREGRINA, ON GROWTH OF ESCHERICHIA-COLI [J].

ANDO, K ;

NATORI, S .

JOURNAL OF BIOCHEMISTRY, 1988, 103 (04) :735-739

[2] PURIFICATION OF SARCOTOXIN-II, ANTIBACTERIAL PROTEINS OF SARCOPHAGA-PEREGRINA (FLESH FLY) LARVAE [J].

ANDO, K ;

OKADA, M ;

NATORI, S .

BIOCHEMISTRY, 1987, 26 (01) :226-230

[3] MOLECULAR-CLONING, SEQUENCING, AND CHARACTERIZATION OF CDNA FOR SARCOTOXIN-IIA, AN INDUCIBLE ANTIBACTERIAL PROTEIN OF SARCOPHAGA-PEREGRINA (FLESH FLY) [J].

ANDO, K ;

NATORI, S .

BIOCHEMISTRY, 1988, 27 (05) :1715-1721

[4] PURIFICATION OF SARCOTOXIN-III, A NEW ANTIBACTERIAL PROTEIN OF SARCOPHAGA-PEREGRINA [J].

BABA, K ;

OKADA, M ;

KAWANO, T ;

KOMANO, H ;

NATORI, S .

JOURNAL OF BIOCHEMISTRY, 1987, 102 (01) :69-74

[5]

Boman H.G., 1986, P63

[6] CELL-FREE IMMUNITY IN CECROPIA - A MODEL SYSTEM FOR ANTIBACTERIAL PROTEINS [J].

BOMAN, HG ;

FAYE, I ;

GUDMUNDSSON, GH ;

LEE, JY ;

LIDHOLM, DA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 201 (01) :23-31

[7]

BOMAN HG, 1987, ANNU REV MICROBIOL, V41, P103, DOI 10.1146/annurev.mi.41.100187.000535

[8] ANTIBACTERIAL PEPTIDES - KEY COMPONENTS NEEDED IN IMMUNITY [J].

BOMAN, HG .

CELL, 1991, 65 (02) :205-207

[9] INSECT IMMUNITY - PURIFICATION AND CHARACTERIZATION OF A FAMILY OF NOVEL INDUCIBLE ANTIBACTERIAL PROTEINS FROM IMMUNIZED LARVAE OF THE DIPTERAN PHORMIA-TERRANOVAE AND COMPLETE AMINO-ACID-SEQUENCE OF THE PREDOMINANT MEMBER, DIPTERICIN-A [J].

DIMARCQ, JL ;

KEPPI, E ;

DUNBAR, B ;

LAMBERT, J ;

REICHHART, JM ;

HOFFMANN, D ;

RANKINE, SM ;

FOTHERGILL, JE ;

HOFFMANN, JA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 171 (1-2) :17-22

[10] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

← 1 2 3 →