THE LIPID BODY LIPOXYGENASE FROM CUCUMBER SEEDLINGS EXHIBITS UNUSUAL REACTION SPECIFICITY

被引：32

作者：

FEUSSNER, I ^{[1
]}

KUHN, H ^{[1
]}

机构：

[1] HUMBOLDT UNIV BERLIN,UNIV KLINIKUM CHARITE,INST BIOCHEM,D-10115 BERLIN,GERMANY

来源：

FEBS LETTERS | 1995年 / 367卷 / 01期

关键词：

CUCUMIS SATIVUS; LIPID BODY; LIPOXYGENASE; POSITIONAL SPECIFICITY; MEMBRANE BINDING;

D O I：

10.1016/0014-5793(95)00531-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipid body lipoxygenase of cucumber seedlings is at a high level expressed during the germinating process which is the stage of triglyceride mobilisation. This enzyme exhibits an unusual positional specificity which has not been described so far for any plant and animal lipoxygenase. The purified enzyme converts arachidonic acid to 15-S-hydroperoxy5Z,8Z,11Z, 13E-eicosatetraenoic acid (15S-HPETE), 12-S-hydroperoxy-5Z, 8Z, 10E, 14Z-eicosatetraenoic acid (12S-HPETE), and 8-S-hydroperoxy-5Z,9E,11Z,14Z-eicosatetraenoic acid (8S-HPETE) in a ratio of 76:4:20 with the corresponding R-isomers being only minor contaminants. Binding to the lipid bodies enhances the arachidonic acid dioxygenase activity more than 4-times and alters positional specificity of the enzyme in favour of 8-S-hydroperoxy-5Z,9E,11Z,14Z-eicosatetraenoic acid (8S-HPETE) formation.

引用

页码：12 / 14

页数：3

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