DROSOPHILA WING-SPOT TEST - IMPROVED DETECTABILITY OF GENOTOXICITY OF POLYCYCLIC AROMATIC-HYDROCARBONS

被引:56
作者
FROLICH, A
WURGLER, FE
机构
[1] SWISS FED INST TECHNOL,INST TOXICOL,SCHORENSTR 16,CH-8603 SCHWERZENBACH,SWITZERLAND
[2] UNIV ZURICH,CH-8603 SCHWERZENBACH,SWITZERLAND
来源
MUTATION RESEARCH | 1990年 / 234卷 / 02期
关键词
7,12-Dimethylbenz[a]anthracene; Benzo[a]pyrene; Benz[a]anthracene; Cytochrome P-450; Drosophila; Genotoxicity; Metabolic activation; Promutagens;
D O I
10.1016/0165-1161(90)90033-K
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The somatic mutation and recombination tests (SMART) using eyes or wings in the fruit fly Drosophila melanogaster are flexible and sensitive systems for the detection of genotoxicity of individual chemical compounds and complex mixtures. It is of special interest that adults and larvae possess cytochrome P-450-dependent activation systems able to metabolize most promutagens, e.g., nitrosamines, aflatoxins, pyrrolizidine alkaloids, safrole, etc. The polycyclic aromatic hydrocarbons (PAHs) represent a class of promutagens poorly detectable in Drosophila genotoxicity systems. Therefore, new tester strains for the wing-spot test were constructed by introducing chromosomes 1 and 2 from a wild-type strain with increased cytochrome P-450-dependent metabolism linked to a gene on chromosome 2. Previous investigations with the new strains showed their increased detection capability for diethylnitrosamine. Comparative tests with the 3 PAHs benzo[a]pyrene, benz[a]anthracene and 7,12-dimethylbenz[a]anthracene demonstrate, in a reproducible way, that with the new strains all 3 can be detected as active genotoxic compounds. The dose-response curves for all compounds show a plateau with higher exposures. This is interpreted as indicative of a saturation of the cytochrome P-450-dependent activation systems. © 1990.
引用
收藏
页码:71 / 80
页数:10
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