MIXED AGGLUTINATION IN MICROTITER U-TRAYS

The principle of mixed agglutination with cell cultures was applied to cells which could not be obtained as monolayer cell cultures. Artifical monolayers of freshly isolated cells from various tissues were prepared in poly-L-lysine-coated wells of a microtiter U-tray. By means of this technique, murine alloantigens, H-2 private specificities and Thy-1 antigens were demonstrated on cells from thymus, brain, spleen, kidney and liver. Ia.2 antigen was detected on splenocytes but not on thymocytes or fibroblast L cells. Evidence was also presented that HLA A and B locus antigens could be demonstrated on human thymocytes. Sensitivity of this test was at least 100 times higher than that of the standard lymphocytotoxicity test for detection of HLA antigens.