PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR BOVINE GAMMA-INTERFERON

被引：48

作者：

WOOD, PR ^{[1
]}

ROTHEL, JS ^{[1
]}

MCWATERS, PGD ^{[1
]}

JONES, SL ^{[1
]}

机构：

[1] COMMONWEALTH SERUM LAB,PARKVILLE,VIC 3052,AUSTRALIA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1990年 / 25卷 / 01期

关键词：

D O I：

10.1016/0165-2427(90)90108-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Nine stable hybridoma cell lines were established which secreted specific monoclonal antibodies (MAbs) to bovine gamma-interferon (BoIFN-γ). Specific binding of each of the MAbs to recombinant BoIFN-γ (rBoIFN-γ) was demonstrated in an indirect ELISA, whilst none of the MAbs bound to rBoIFN-α or rBoIFN-β. In a Western blot the MAbs reacted with the 16 kDa and 32 kDa polypeptides present in rBoIFN-γ preparations. Competitive ELISA's showed that four MAbs bound to one epitope on rBoIFN-γ, and the other five MAbs bound to a separate epitope. Two MAbs, each recognising different epitopes, were shown to neutralise the anti-viral activity of natural BoIFN-γ. © 1990.

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页码：37 / 46

页数：10

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