IDENTIFICATION OF COOPERATING ONCOGENES IN E-MU-MYC TRANSGENIC MICE BY PROVIRUS TAGGING

被引：672

作者：

VANLOHUIZEN, M ^{[1
]}

VERBEEK, S ^{[1
]}

SCHEIJEN, B ^{[1
]}

WIENTJENS, E ^{[1
]}

VANDERGULDEN, H ^{[1
]}

BERNS, A ^{[1
]}

机构：

[1] UNIV AMSTERDAM,DEPT BIOCHEM,AMSTERDAM,NETHERLANDS

来源：

CELL | 1991年 / 65卷 / 05期

关键词：

D O I：

10.1016/0092-8674(91)90382-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mo-MLV infection of E-mu-myc transgenic mice results in a dramatic acceleration of pre-B cell lymphomagenesis. We have used provirus tagging to identify genes that cooperate with the E-mu-myc transgene in B cell transformation. Here we report on the identification of four loci, pim-1, bmi-1, pal-1, and bla-1, which are occupied by proviruses in 35%, 35%, 28%, and 14% of the tumors, respectively. bmi-1, pal-1, and bla-1 represent novel common proviral insertion sites. The bmi-1 gene encodes a 324 amino acid protein with a predominantly nuclear localization. bmi-1 is highly conserved in evolution and contains several motifs frequently found in transcriptional regulators, including a new putative zinc finger motif. No genes have yet been assigned to pal-1 and bla-1. The distribution of proviruses over the four common insertion sites suggests that provirus tagging can be used not only to identify the cooperating oncogenes but also to assign these genes to distinct complementation groups in tumorigenesis.

引用

页码：737 / 752

页数：16

共 79 条

[1] THE C-MYC ONCOGENE DRIVEN BY IMMUNOGLOBULIN ENHANCERS INDUCES LYMPHOID MALIGNANCY IN TRANSGENIC MICE [J].