UNEXPECTEDLY HIGHER EXPRESSION LEVELS OF A CHIMERIC 2S ALBUMIN SEED PROTEIN TRANSGENE FROM A TANDEM ARRAY CONSTRUCT

被引：9

作者：

CONCEICAO, AD ^{[1
]}

VANVLIET, A ^{[1
]}

KREBBERS, E ^{[1
]}

机构：

[1] PLANT GENET SYST NV, B-9000 GHENT, BELGIUM

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 03期

关键词：

25; ALBUMIN; ARABIDOPSIS; BRAZIL NUT; POSTTRANSCRIPTIONAL REGULATION; TANDEM GENES; TRANSGENIC PLANT;

D O I：

10.1007/BF00028867

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expression levels were obtained using a construct in which the transgene was present as a tandem duplication in the T-DNA. Neither in this case nor in homozygous plants with either construct was epigenetic silencing observed. While transgene mRNA levels were of the same order of magnitude as the endogenous at2S2 gene, protein levels were much lower.

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页码：1001 / 1005

页数：5

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