THE PRO SEQUENCE OF LACTASE-PHLORHIZIN HYDROLASE IS REQUIRED FOR THE ENZYME TO REACH THE PLASMA-MEMBRANE - AN INTRAMOLECULAR CHAPERONE

被引：28

作者：

OBERHOLZER, T ^{[1
]}

MANTEI, N ^{[1
]}

SEMENZA, G ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,DEPT BIOCHEM,CH-8092 ZURICH,SWITZERLAND

来源：

FEBS LETTERS | 1993年 / 333卷 / 1-2期

关键词：

SORTING; TARGETING; PRO SEQUENCE; LACTASE-PHLORHIZIN HYDROLASE; PLASMA MEMBRANE; PROTEOLYTIC PROCESSING;

D O I：

10.1016/0014-5793(93)80389-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro sequence and the lactase domain of mature LPH, accumulated intracellularly; the pro sequence with no mature domain was secreted. We conclude that the pro sequence is important for LPH to be transported to the cell surface.

引用

页码：127 / 131

页数：5

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