CLONING AND SEQUENCING A PUTATIVE PYROPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASE GENE FROM ENTAMOEBA-HISTOLYTICA

被引：12

作者：

HUANG, M

ALBACH, RA

CHANG, KP

TRIPATHI, RL

KEMP, RG

机构：

[1] CHICAGO MED SCH,DEPT MICROBIOL,N CHICAGO,IL 60064

[2] CHICAGO MED SCH,DEPT IMMUNOL & BIOL CHEM,N CHICAGO,IL 60064

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1260卷 / 02期

关键词：

GENE STRUCTURE; PHOSPHOFRUCTOKINASE; (ENTAMOEBA-HISTOLYTICA);

D O I：

10.1016/0167-4781(94)00216-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyrophosphate-dependent phosphofructokinase (PPi-PFK) gene from Entamoeba histolytica was cloned from its genomic library and sequenced. The open reading frame has 1149 bp and codes for a protein of 41.5 kDa. The deduced amino acid sequence of E. histolytica PPi-PFK has 25 to 28% identity to the PPi-PFKs from Propionibacterium freudenreichii, Naegleria fowleri and potato. The amino acid residues known to contribute to the active site of PPi-PFK from P. freudenreichii are conserved.

引用

页码：215 / 217

页数：3

共 11 条

[1] KINETIC MECHANISM OF PYROPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASE FROM PROPIONIBACTERIUM-FREUDENREICHII [J].

BERTAGNOLLI, BL ;

COOK, PF .

BIOCHEMISTRY, 1984, 23 (18) :4101-4108

[2] UNUSUAL GENE ORGANIZATION IN THE PROTOZOAN PARASITE ENTAMOEBA-HISTOLYTICA [J].

BRUCHHAUS, I ;

LEIPPE, M ;

LIOUTAS, C ;

TANNICH, E .

DNA AND CELL BIOLOGY, 1993, 12 (10) :925-933

[3] MOLECULAR-PROPERTIES OF PYROPHOSPHATE - FRUCTOSE-6-PHOSPHATE PHOSPHOTRANSFERASE FROM POTATO-TUBER [J].

KRUGER, NJ ;

DENNIS, DT .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 256 (01) :273-279

[4]

LADROR US, 1991, J BIOL CHEM, V266, P16550

[5] PRESENCE OF A FRUCTOSE-2,6-BISPHOSPHATE-INSENSITIVE PYROPHOSPHATE - FRUCTOSE-6-PHOSPHATE PHOSPHOTRANSFERASE IN THE ANAEROBIC PROTOZOA TRITRICHOMONAS-FOETUS, TRICHOMONAS-VAGINALIS AND ISOTRICHA-PROSTOMA [J].

MERTENS, E ;

VANSCHAFTINGEN, E ;

MULLER, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 37 (02) :183-190

[6] OCCURRENCE OF PYROPHOSPHATE - FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE IN GIARDIA-LAMBLIA TROPHOZOITES [J].

MERTENS, E .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 40 (01) :147-149

[7] PYROPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASE FROM THE AMEBA NAEGLERIA-FOWLERI, AN AMP-SENSITIVE ENZYME [J].

MERTENS, E ;

DEJONCKHEERE, J ;

VANSCHAFTINGEN, E .

BIOCHEMICAL JOURNAL, 1993, 292 :797-803

[8] PURIFICATION AND PROPERTIES OF A PYROPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASE FROM TOXOPLASMA-GONDII [J].

PENG, ZY ;

MANSOUR, TE .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 54 (02) :223-230

[9]

REEVES RE, 1984, ADV PARASIT, V23, P105, DOI 10.1016/S0065-308X(08)60286-9

[10]

REEVES RE, 1974, J BIOL CHEM, V249, P7737

← 1 2 →