ISOPYCNIC SEPARATION OF SUBCELLULAR COMPONENTS FROM POLIOVIRUS-INFECTED AND NORMAL HELA CELLS

被引：146

作者：

BALTIMORE, D

HUANG, AS

机构：

[1] Department of Biology, Massachusetts Institute of Technology, Cambridge

来源：

SCIENCE | 1968年 / 162卷 / 3853期

关键词：

D O I：

10.1126/science.162.3853.572

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A rapid method for determining the density of ribonucleoprotein particles and complexes has been developed. The method involves glutaraldehyde fixation of sucrose gradient fractions and immediate centrifugation for 5 hours through preformed cesium chloride gradients. There is little or no aggregation of particles, so that components which co-sediment in sucrose gradients are resolved by the cesium chloride gradient. By this method the densities of HeLa cell ribosomes, polyribosomes, and subribosomal particles have been determined. Furthermore, the poliovirus replication complex has been separated from polyribosomes and its density has been founid to diaminetetraacetic acid.

引用

页码：572 / +

页数：1

共 13 条

[1] ASPECTS OF SYNTHESIS OF POLIOVIRUS RNA AND FORMATION OF VIRUS PARTICLES [J].