CYTOCHROME-P-450-DEPENDENT VASODILATION OF RAT-KIDNEY BY ARACHIDONIC-ACID

被引：32

作者：

OYEKAN, AO ^{[1
]}

MCGIFF, JC ^{[1
]}

QUILLEY, J ^{[1
]}

机构：

[1] NEW YORK MED COLL,DEPT PHARMACOL,VALHALLA,NY 10595

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 03期

关键词：

INDUCTION; DEPLETION OF CYTOCHROME-P-450; SUPERFUSION BIOASSAY; ARACHIDONIC ACID METABOLISM;

D O I：

10.1152/ajpheart.1991.261.3.H714

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Our previous studies indicated a role for cytochrome P-450-dependent enzymes in generating the mediators of the vasodilator effect of arachidonic acid (AA) in the preconstricted indomethacin-treated perfused kidney of the rat. We report that in vivo induction of cytochrome P-450 enzymes with 3-methylcholanthrene-beta-naphthoflavone or dexamethasone enhanced the renal vasodilator effect of AA in this experimental preparation. Conversely, depletion of cytochrome P-450 enzymes with stannous chloride or cobalt chloride diminished the vasodilator response to AA. Injection of AA resulted in the release of relaxant material into the renal effluent detected by superfusion of rabbit aortic rings. Inhibition of cytochrome P-450 with 7-ethoxyresorufin reduced the release of vasorelaxant material. Metabolism of labeled AA by the kidney revealed four peaks of radioactivity that were recovered from the renal effluent. The heights of these peaks were reduced by 7-ethoxyresorufin. These results provide further evidence for cytochrome P-450-dependent metabolism of AA to one or more vasodilator products by the rat kidney.

引用

页码：H714 / H719

页数：6

共 19 条

[1] 5,6-EPOXYEICOSATRIENOIC ACID, A NOVEL ARACHIDONATE METABOLITE - MECHANISM OF VASOACTIVITY IN THE RAT [J].

CARROLL, MA ;

GARCIA, MP ;

FALCK, JR ;

MCGIFF, JC .

CIRCULATION RESEARCH, 1990, 67 (05) :1082-1088

[2]

ERMAN A, 1986, J PHARMACOL EXP THER, V239, P296

[3]

ESCALANTE B, 1900, ADV PROSTAGLANDIN TH, V21, P204

[4]

FERRERI NR, 1984, J PHARMACOL EXP THER, V231, P441

[5]

FITZPATRICK FA, 1989, PHARMACOL REV, V40, P229

[6] HEMODYNAMIC EFFECTS OF PROSTAGLANDINS IN THE RAT - EVIDENCE FOR IMPORTANT SPECIES VARIATION IN RENOVASCULAR RESPONSES [J].

GERBER, JG ;

NIES, AS .

CIRCULATION RESEARCH, 1979, 44 (03) :406-410

[7]

HARRIS RC, 1989, KIDNEY INT, V35, pA312

[8]

JACOBSON HR, 1985, PROSTAGLANDINS MEMBR, P311

[9]

MAINES MD, 1975, J BIOL CHEM, V250, P4171

[10]

OYEKAN A, 1990, FASEB Journal, V4, pA606

← 1 2 →