AN ULTRAVIOLET LIGHT-INDUCED CROSS-LINK IN YEAST TRANSFER RNAPHE

被引：32

作者：

BEHLEN, LS ^{[1
]}

SAMPSON, JR ^{[1
]}

UHLENBECK, OC ^{[1
]}

机构：

[1] UNIV COLORADO,DEPT CHEM & BIOCHEM,BOULDER,CO 80309

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 15期

关键词：

D O I：

10.1093/nar/20.15.4055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The irradiation of native or unmodified yeast tRNA(Phe) by short wavelength UV light (260 nM) results in an intramolecular crosslink that has been mapped to occur between C48 in the variable loop and U59 in the T loop. Photo-reversibility of the crosslink and the absence of fluorescent photo adducts suggest that the crosslink product is a cytidine-uridine cyclobutane dimer. This is consistent with the relative geometries of C48 and U59 in the crystal structure of yeast tRNA(Phe). Evaluation of the crosslinking efficiency of the mutants of tRNA(Phe) indicates that the reaction depends on the correct tertiary structure of the RNA.

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页码：4055 / 4059

页数：5

相关论文

共 27 条

[1] INVESTIGATION OF THE TERTIARY FOLDING OF ESCHERICHIA-COLI 16S RNA BY INSITU INTRA-RNA CROSS-LINKING WITHIN 30S RIBOSOMAL-SUBUNITS [J].

ATMADJA, J ;

BRIMACOMBE, R ;

BLOCKER, H ;

FRANK, R .

NUCLEIC ACIDS RESEARCH, 1985, 13 (19) :6919-6936

[2] LEAD-CATALYZED CLEAVAGE OF YEAST TRANSFER RNAPHE MUTANTS [J].

BEHLEN, LS ;

SAMPSON, JR ;

DIRENZO, AB ;

UHLENBECK, OC .

BIOCHEMISTRY, 1990, 29 (10) :2515-2523

[3] PHOTOREACTION OF 4-THIOURACIL WITH CYTOSINE - RELATION TO PHOTOREACTIONS IN ESCHERICHIA-COLI TRANSFER RIBONUCLEIC-ACIDS [J].

BERGSTROM, DE ;

LEONARD, NJ .

BIOCHEMISTRY, 1972, 11 (01) :1-+

[4] ULTRAVIOLET LIGHT-INDUCED CROSSLINKING REVEALS A UNIQUE REGION OF LOCAL TERTIARY STRUCTURE IN POTATO SPINDLE TUBER VIROID AND HELA 5S RNA [J].

BRANCH, AD ;

BENENFELD, BJ ;

ROBERTSON, HD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (19) :6590-6594

[5] UV-INDUCED MUTATION HOTSPOTS OCCUR AT DNA DAMAGE HOTSPOTS [J].

BRASH, DE ;

HASELTINE, WA .

NATURE, 1982, 298 (5870) :189-192

[6]

Brown Daniel R.L., 2009, IACR CRYPTOL EPRINT, V2009, P620

[7] CRYSTALLOGRAPHIC AND BIOCHEMICAL INVESTIGATION OF THE LEAD(II)-CATALYZED HYDROLYSIS OF YEAST PHENYLALANINE TRANSFER-RNA [J].

BROWN, RS ;

DEWAN, JC ;

KLUG, A .

BIOCHEMISTRY, 1985, 24 (18) :4785-4801

[8] AN ULTRAVIOLET-INDUCIBLE ADENOSINE ADENOSINE CROSS-LINK REFLECTS THE CATALYTIC STRUCTURE OF THE TETRAHYMENA RIBOZYME [J].

DOWNS, WD ;

CECH, TR .

BIOCHEMISTRY, 1990, 29 (23) :5605-5613

[9] 3'-TERMINAL LABELING OF RNA WITH T4 RNA LIGASE [J].

ENGLAND, TE ;

UHLENBECK, OC .

NATURE, 1978, 275 (5680) :560-561

[10] PHOTOCHEMISTRY OF 4-THOURIDINE IN ESCHERICHIA COLI T-RNA1.VAL [J].

FAVRE, A ;

YANIV, M ;

MICHELSO.AM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 37 (02) :266-&