COUPLING OF D2 DOPAMINE-RECEPTORS AND D3 DOPAMINE-RECEPTORS TO G-PROTEINS

被引：65

作者：

CASTRO, SW ^{[1
]}

STRANGE, PG ^{[1
]}

机构：

[1] UNIV CANTERBURY,BIOL LAB,CANTERBURY CT2 7NJ,KENT,ENGLAND

来源：

FEBS LETTERS | 1993年 / 315卷 / 03期

关键词：

D2 DOPAMINE RECEPTOR; D3 DOPAMINE RECEPTOR; G-PROTEIN; AGONIST BINDING;

D O I：

10.1016/0014-5793(93)81168-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[H-3]spiperone competition experiments. D2(short) expressed in Ltk- cells, D2(long) expressed in Ltk- or CHO cells and D3 expressed in CHO cells all showed coupling to the endogenous G-proteins of the cells. The detailed agonist binding characteristics indicated that there were differences in receptor/G-protein coupling for the same receptor (D2(long)) expressed in two cell types or for different receptors (D2(short), D2(long)) expressed in the same cell type.

引用

页码：223 / 226

页数：4

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