NEW METHOD FOR THE DETERMINATION OF C-14 RADIOACTIVITY IN KETONE BODIES

被引：9

作者：

MEWES, W

SEUFERT, CD

SOLING, HD

机构：

[1] Abt. für Klinische Biochemie, Medizinische Universitätsklinik Göttingen, Humboldtalle 1

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90677-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reaction vessel is described consisting of a simple reaction chamber fixed to an ordinary liquid scintillation-counting vial which allows the direct determination of radioactivity in acetone as well as separately in the carboxyl and acetone moieties of acetoacetate and 3-hydroxybutyrate. Radioactivity and its distribution between carbon 1 and carbons 2 through 4 can be determined in total ketone bodies as well as sequentially in acetone, acetoacetate, and 3-hydroxybutyrate. Recoveries with labeled ketone bodies under a variety of analytical conditions are presented. © 1979.

引用

页码：394 / 402

页数：9

共 14 条

[1] EVIDENCE FOR AN EFFECT OF INSULIN ON PERIPHERAL UTILIZATION OF KETONE BODIES IN DOGS [J].