DETECTION OF A SPECIFIC MITOCHONDRIAL-DNA DELETION IN TISSUES OF OLDER HUMANS

被引：672

作者：

CORTOPASSI, GA

ARNHEIM, N

机构：

[1] Molecular Biology Section, University of Southern California, Los Angeles

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 23期

关键词：

D O I：

10.1093/nar/18.23.6927

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using PCR, we found that normal heart muscle and brain from adult human individuals contain low levels of a specific mitochondrial DNa deletion, previously found only in patients affected with certain types of neuromuscular disease. This deletion was not observed in fetal heart or brain. Experimental tests support the idea that the deletion exists in vivo in adult mitochondria and is not an in vitro artifact of PCR. Our data provide direct experimental support for the idea that accumulation of mitochondrial DNA deletions may be important in aging.

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页码：6927 / 6933

页数：7

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