CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE LACTOSE-SPECIFIC PHOSPHOCARRIER PROTEIN-IIALAC OF THE PHOSPHOENOLPYRUVATE - SUGAR PHOSPHOTRANSFERASE SYSTEM FROM STAPHYLOCOCCUS-AUREUS

被引：6

作者：

CELIKEL, R

DAI, XP

STEWART, GC

SUTRINA, SL

SAIER, MH

XUONG, NH

REIZER, J

机构：

[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

[2] UNIV KANSAS,DEPT MICROBIOL,LAWRENCE,KS 66045

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 04期

关键词：

X-RAY ANALYSIS; ENZYME-IIA (LACTOSE); ENZYME-III (LACTOSE); SUGAR PHOSPHOTRANSFERASE SYSTEM;

D O I：

10.1016/0022-2836(91)90576-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The IIA constituent of the lactose permease from Staphylococcus aureus has been crystallized in two different forms. Crystals of form I have been grown from polyethylene glycol 4000 with β-octyl glucoside. They diffract to 3·0 Å resolution and belong to space group C2 with unit cell dimensions a = 141·7 A ̊, b = 130·7 A ̊, c = 96·5 A ̊ and β = 96·2 °· Form II crystals have been obtained from a solution containing polyethylene glycol 400, ammonium sulfate and manganese chloride. They diffract to at least 2·8 Å resolution and belong to space group P212121 with unit cell dimensions a = 89·9 A ̊, b = 101·5 A ̊ and c = 90·9 A ̊. © 1991.

引用

页码：857 / 859

页数：3

共 15 条

[1]

BREIDT F, 1987, J BIOL CHEM, V262, P16444

[2] PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF STAPHYLOCOCCUS-AUREUS - FACTOR-IIILAC, A TRIMERIC PHOSPHO-CARRIER PROTEIN THAT ALSO ACTS AS A PHASE-TRANSFER CATALYST [J].