CYTOGENETIC AND MOLECULAR ANALYSIS OF A MASKED PHILADELPHIA-CHROMOSOME IN CHRONIC AND BLASTIC PHASES OF CHRONIC MYELOID-LEUKEMIA

被引：7

作者：

ZITZELSBERGER, H

BAUCHINGER, M

WILMANNS, W

STRAUSS, PG

机构：

[1] GESELL STRAHLEN & UMWELTFORSCH MBH,INST STRAHLENBIOL,ARBEITSGRP ZYTOGENET,W-8042 NEUHERBERG,GERMANY

[2] UNIV MUNICH,KLINIKUM GROSSHADERN,MED KLIN 3,W-8000 MUNICH 70,GERMANY

[3] GESELL STRAHLEN & UMWELTFORSCH MBH,MOLEK ZELLPATHOL ABT,W-8042 NEUHERBERG,GERMANY

来源：

CANCER GENETICS AND CYTOGENETICS | 1990年 / 47卷 / 02期

关键词：

D O I：

10.1016/0165-4608(90)90031-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A "masked" Philadelphia chromosome (Ph), t(1;22;9)(p32;q11;q34), was found in the bone marrow and peripheral blood cells of a patient with chronic myeloid leukemia (CML) during the chronic and blastic phases of the disease. As an additional change, a reciprocal translocation t(12;13)(p13;q14) was observed in the blastic phase. Southern blot analysis showed a rearrangement of the breakpoint cluster region (bcr). Northern blot analysis with a c-abl probe showed an abnormal 8.5 kb c-abl RNA transcript in addition to the normal 6- and 7-kilobase (kb) c-abl species. Thus, the results demonstrate the presence of a c-abl/bcr rearrangement in the masked Ph corresponding to that observed in the standard Ph translocation t(9;22)(q34;q11) of CML. © 1990.

引用

页码：219 / 225

页数：7

共 31 条

[1] THE KARYOTYPE OF BLASTIC CRISIS [J].

ALIMENA, G ;

DECUIA, MR ;

DIVERIO, D ;

GASTALDI, R ;

NANNI, M .

CANCER GENETICS AND CYTOGENETICS, 1987, 26 (01) :39-50

[2] TRANSLOCATION OF C-ABL ONCOGENE CORRELATES WITH THE PRESENCE OF A PHILADELPHIA-CHROMOSOME IN CHRONIC MYELOCYTIC-LEUKEMIA [J].

BARTRAM, CR ;

DEKLEIN, A ;

HAGEMEIJER, A ;

VANAGTHOVEN, T ;

VANKESSEL, AG ;

BOOTSMA, D ;

GROSVELD, G ;

FERGUSONSMITH, MA ;

DAVIES, T ;

STONE, M ;

HEISTERKAMP, N ;

STEPHENSON, JR ;

GROFFEN, J .

NATURE, 1983, 306 (5940) :277-280

[3] C-ABL AND BCR ARE REARRANGED IN A PH1-NEGATIVE CML PATIENT [J].

BARTRAM, CR ;

KLEIHAUER, E ;

DEKLEIN, A ;

GROSVELD, G ;

TEYSSIER, JR ;

HEISTERKAMP, N ;

GROFFEN, J .

EMBO JOURNAL, 1985, 4 (03) :683-686

[4]

BERNSTEIN R, 1984, BLOOD, V63, P399

[5] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[6]

DAVIS LG, 1986, BASIC METHODS MOL BI, P139

[7]

DEKLEIN A, 1986, BLOOD, V68, P1369

[8] A CELLULAR ONCOGENE IS TRANSLOCATED TO THE PHILADELPHIA-CHROMOSOME IN CHRONIC MYELOCYTIC-LEUKEMIA [J].

DEKLEIN, A ;

VANKESSEL, AG ;

GROSVELD, G ;

BARTRAM, CR ;

HAGEMEIJER, A ;

BOOTSMA, D ;

SPURR, NK ;

HEISTERKAMP, N ;

GROFFEN, J ;

STEPHENSON, JR .

NATURE, 1982, 300 (5894) :765-767

[9] AN 8-KILOBASE ABL RNA TRANSCRIPT IN CHRONIC MYELOGENOUS LEUKEMIA [J].

GALE, RP ;

CANAANI, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (18) :5648-5652

[10] PHILADELPHIA CHROMOSOMAL BREAKPOINTS ARE CLUSTERED WITHIN A LIMITED REGION, BCR, ON CHROMOSOME-22 [J].

GROFFEN, J ;

STEPHENSON, JR ;

HEISTERKAMP, N ;

DEKLEIN, A ;

BARTRAM, CR ;

GROSVELD, G .

CELL, 1984, 36 (01) :93-99

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