ENERGETICS OF B-Z JUNCTION FORMATION IN A 16 BASE-PAIR DUPLEX DNA

被引：19

作者：

DOKTYCZ, MJ ^{[1
]}

BENIGHT, AS ^{[1
]}

SHEARDY, RD ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT CHEM,HAZLETON,PA 18201

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 01期

关键词：

D O I：

10.1016/0022-2836(90)90297-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report analysis of the NaCl-induced B-Z transition in a 16 base-pair duplex DNA with sequence designed such that when NaCl is increased the left half of the molecule undergoes the B-Z transition while the right half remains in the B-form. An equilibrium thermodynamic model based on the body of available published experimental data and the recent theoretical work of Soumpasis, which indicate, in the salt range above ~0.9 m-NaCl, the transition free-energy of B-Z conversion in DNA is a linear function of the NaCl concentration, is employed. Analysis of the B-Z transition of the junction-containing molecule indicates the B-Z junction formed in this 16 base-pair DNA is composed of approximately three base-pairs and has a free energy of formation of ~4.7 kcal/mol junction. These values for the junction are in excellent agreement with published estimates of B-Z junction size and energy derived from much longer DNA pieces. © 1990.

引用

页码：3 / 6

页数：4

共 18 条

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CARUTHERS MH, 1982, CHEM ENZYMATIC SYNTH

[2] COVALENT ATTACHMENT OF AN ALKYLAMINE PREVENTS THE B TO Z TRANSITION IN POLY(DG-DC) [J].