GENETIC-TRANSFORMATION OF MEDICAGO-TRUNCATULA USING AGROBACTERIUM WITH GENETICALLY MODIFIED RI AND DISARMED TI PLASMIDS

被引：5

作者：

THOMAS, MR ^{[1
]}

ROSE, RJ ^{[1
]}

NOLAN, KE ^{[1
]}

机构：

[1] UNIV NEWCASTLE, DEPT BIOL SCI, NEWCASTLE, NSW 2308, AUSTRALIA

来源：

PLANT CELL REPORTS | 1992年 / 11卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Fertile transgenic plants of the annual pasture legume Medicago truncatula were obtained by Agrobacterium-mediated transformation, utilising a disarmed Ti plasmid and a binary vector containing the kanamycin resistance gene under the control of the cauliflower mosaic virus 35S promoter. Factors contributing to the result included an improved plant regeneration protocol and the use of explants from a plant identified as possessing high regeneration capability from tissue culture. Genes present on the T-DNA of the Ri plasmid had a negative effect on somatic embryogenesis. Only tissue inoculated with Agrobacterium strains containing a disarmed Ti plasmid lacking the T-DNA region or a Ri plasmid with an inactivated rol A gene regenerated transgenic plants. Fertile transgenic plants were only obtained with disarmed A. tumefaciens, and the introduced NPT II gene was transmitted to R1 progeny.

引用

页码：113 / 117

页数：5

共 21 条

[1] CHANGES IN T-DNA METHYLATION AND EXPRESSION ARE ASSOCIATED WITH PHENOTYPIC VARIATION AND PLANT-REGENERATION IN A CROWN GALL TUMOR LINE [J].

AMASINO, RM ;

POWELL, ALT ;

GORDON, MP .

MOLECULAR & GENERAL GENETICS, 1984, 197 (03) :437-446

[2]

Barker D. G., 1990, Plant Molecular Biology Reporter, V8, P40, DOI 10.1007/BF02668879

[3]

CRAWFORD EJ, 1989, ADV AGRON, V42, P399

[4] TRANSFORMATION OF MEDICAGO BY AGROBACTERIUM MEDIATED GENE-TRANSFER [J].

DEAK, M ;

KISS, GB ;

KONCZ, C ;

DUDITS, D .

PLANT CELL REPORTS, 1986, 5 (02) :97-100

[5] STABLE TRANSFORMATION OF MAIZE AFTER GENE-TRANSFER BY ELECTROPORATION [J].

FROMM, ME ;

TAYLOR, LP ;

WALBOT, V .

NATURE, 1986, 319 (6056) :791-793

[6] THE HYPERVIRULENCE OF AGROBACTERIUM-TUMEFACIENS A281 IS ENCODED IN A REGION OF PTIBO542 OUTSIDE OF TRANSFER DNA [J].

HOOD, EE ;

HELMER, GL ;

FRALEY, RT ;

CHILTON, MD .

JOURNAL OF BACTERIOLOGY, 1986, 168 (03) :1291-1301

[7] HAIRY-ROOT-INDUCING PLASMID - PHYSICAL MAP AND HOMOLOGY TO TUMOR-INDUCING PLASMIDS [J].

HUFFMAN, GA ;

WHITE, FF ;

GORDON, MP ;

NESTER, EW .

JOURNAL OF BACTERIOLOGY, 1984, 157 (01) :269-276

[8] GENETIC-ANALYSIS OF THE INDIVIDUAL T-DNA GENES OF AGROBACTERIUM-TUMEFACIENS - FURTHER EVIDENCE THAT 2 GENES ARE INVOLVED IN INDOLE-3-ACETIC-ACID SYNTHESIS [J].

INZE, D ;

FOLLIN, A ;

VANLIJSEBETTENS, M ;

SIMOENS, C ;

GENETELLO, C ;

VANMONTAGU, M ;

SCHELL, J .

MOLECULAR & GENERAL GENETICS, 1984, 194 (1-2) :265-274

[9] RESTRICTION MAP OF AN AGROPINE-TYPE RI-PLASMID AND ITS HOMOLOGIES WITH TI-PLASMIDS [J].

JOUANIN, L .

PLASMID, 1984, 12 (02) :91-102

[10] STRUCTURE OF T-DNA IN PLANTS REGENERATED FROM ROOTS TRANSFORMED BY AGROBACTERIUM-RHIZOGENES STRAIN-A4 [J].

JOUANIN, L ;

GUERCHE, P ;

PAMBOUKDJIAN, N ;

TOURNEUR, C ;

DELBART, FC ;

TOURNEUR, J .

MOLECULAR & GENERAL GENETICS, 1987, 206 (03) :387-392

← 1 2 3 →