ENZYMATIC-SYNTHESIS OF MANNOBIOSES AND MANNOTRIOSES BY REVERSE HYDROLYSIS USING ALPHA-MANNOSIDASE FROM ASPERGILLUS-NIGER

被引：29

作者：

AJISAKA, K

MATSUO, I

ISOMURA, M

FUJIMOTO, H

SHIRAKABE, M

OKAWA, M

机构：

[1] Meiji Institute of Health Science, Meiji Milk Products Co, Ltd., Odawara, 250

来源：

CARBOHYDRATE RESEARCH | 1995年 / 270卷 / 02期

关键词：

MANNOBIOSES; MANNOTRIOSES; ALPHA-MANNOSIDASE; ASPERGILLUS NIGER;

D O I：

10.1016/0008-6215(95)00015-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various manno-oligosaccharides including alpha-D-man-(1-->2)-D-man and alpha-D-man-(1-->2)-alpha-D-man-(1-->2)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of alpha-mannosidase from Aspergillus niger. alpha-D-Man-(1-->2)-D-man and alpha-D-man-(1-->2)-alpha-D-man-(1-->2)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column. In addition to the above oligosaccharides, alpha-D-man-(1-->3)-D-man, alpha-D-man-(1 -->6)-D-man, and alpha-D-man-(1-->2)-alpha-D-man-(1-->6)-D-man were also isolated.

引用

页码：123 / 130

页数：8

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