INDUCTION OF DNA SYNTHESIS BY ADENOVIRUSES IN CONTACT-INHIBITED HAMSTER CELLS

Highly oncogenic (types 12 and 31), weakly oncogenic (types 3 and 2), and non-oncogenic (types 2 and 5) adenoviruses induced DNA synthesis in contact-inhibited hamster cells. DNA's induced by adenoviruses 2, 7, and 12 in contact-inhibited hamster cells were mainly cellular. High multiplicity of infection was necessary for the induction of DNA synthesis. Adenoviruses 12 and 31 were efficient in the induction of DNA synthesis and the patterns of DNA synthesis induced by these viruses were early and of short duration, whereas adenoviruses 3, 7, 2, and 5 were less efficient in the induction of DNA synthesis and the patterns of DNA synthesis induced by adenoviruses 3 and 7 were late and long-lasting. The patterns of DNA synthesis induced by adenoviruses 2 and 5 were intermediate between these two patterns. Adenoviruses 2 and 5 replicated in hamster cells, and viral DNA detected in DNA induced in hamster cells by adenovirus 2. Adenoviruses 12, 31, 3, and 7 did not replicate in hamster cells and viral DNA's were hardly detected in DNA's induced in hamster cells by adenoviruses 12 and 7. © 1968.