SOME OBSERVATIONS ON PHYSICOCHEMICAL PROPERTIES OF HEMOGLOBIN MHYDE PARK

被引：32

作者：

HAYASHI, A

SUZUKI, T

SHIMIZU, A

IMAI, K

MORIMOTO, H

MIYAJI, T

SHIBATA, S

机构：

[1] The Third Department of Internal Medicine, Osaka University Medical School, Osaka

[2] Department of Physics, Faculty of Engineering Science, Osaka University, Toyonaka, Osaka

[3] The Third Department of Internal Medicine, Yamaguchi University Medical School, Ube

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1968年 / 125卷 / 03期

关键词：

D O I：

10.1016/0003-9861(68)90528-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hemoglobin MHyde Park (Hb MH) and its modified product (Hb MHX) in oxygenated form were obtained by Chromatographic procedures. The patient's blood containing Hb MH revealed EPR spectra with both high and low spin-type signals. In an O2 equilibrium study, Hb MH showed similar functional abnormalities to Hb MSaskatoon, a somewhat higher O2 affinity and a smaller n value than Hb A, and a Bohr effect that was almost normal. But the n value and the O2 affinity of the O2 equilibrium curves varied considerably according to the wavelength used for calculation, suggesting the conformational change of the abnormal subunit of Hb MH. The abnormal subunit of methemoglobin MH was able to react with cyanide, and its K′ value was similar to that of Hb MBoston. Heat-denaturation also disclosed the remarkable instability of Hb MH, which was compatible with the results of the in vivo hematologic study. © 1968.

引用

页码：895 / &

共 13 条

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