INHIBITION OF HOMOSERINE DEHYDROGENASE-I BY L-SERINE IN ESCHERICHIA-COLI

被引:36
作者
HAMA, H [1 ]
KAYAHARA, T [1 ]
TSUDA, M [1 ]
TSUCHIYA, T [1 ]
机构
[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT MICROBIOL,OKAYAMA 700,JAPAN
关键词
D O I
10.1093/oxfordjournals.jbchem.a123427
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have reported that a major cause of growth inhibition of Escherichia coli by L-serine is its inhibition of homoserine dehydrogenase I (HDH I), which is involved in the biosyntheses of threonine and isoleucine [Hama, H., Sumita, Y., Kakutani, Y., Tsuda, M., & Tsuchiya, T. (1990) Biochem. Biophys. Res. Commun. 168, 1211-1216]. However, Patte et al. reported that L-serine does not inhibit HDH I [Patte, J.-C., Truffa-Bachi, P., & Cohen, G.N. (1966) Biochim. Biophys. Acta 128, 426-439]. In studies on the reason for these discrepant results, we found that the concentration of K+ and the pH in the assay mixture strongly influenced the inhibitory effect of L-serine. L-Serine strongly inhibited the HDH I activities in both the forward and reverse reactions between aspartate semialdehyde and homoserine at a physiological K+ concentration (100 to 200 mM) and physiological pH (7.5) for E. coli cells. On the other hand, two well-known inhibitors of HDH I, L-threonine and L-cysteine, strongly inhibited the activity regardless of the K+ concentration and pH.
引用
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页码:604 / 608
页数:5
相关论文
共 30 条
[1]   AMINO ACID UTILIZATION IN BACTERIAL GROWTH .2. A STUDY OF THREONINE-ISOLEUCINE RELATIONSHIPS IN MUTANTS OF ESCHERICHIA-COLI [J].
AMOS, H ;
COHEN, GN .
BIOCHEMICAL JOURNAL, 1954, 57 (02) :338-343
[2]   ASPARTOKINASE HOMOSERINE DEHYDROGENASE-I FROM ESCHERICHIA-COLI - PH AND CHEMICAL MODIFICATION STUDIES OF THE KINASE-ACTIVITY [J].
ANGELES, TS ;
SMANIK, PA ;
BORDERS, CL ;
VIOLA, RE .
BIOCHEMISTRY, 1989, 28 (22) :8771-8777
[3]   BETA-ASPARTYL PHOSPHATE AND ASPARTIC-BETA-SEMIALDEHYDE [J].
BLACK, S .
METHODS IN ENZYMOLOGY, 1963, 6 :622-624
[4]  
COHEN GN, 1987, ESCHERICHIA COLI SAL, P429
[5]   L-SERINE-SENSITIVE MUTANTS OF ESCHERICHIA-COLI-K-12 [J].
COSLOY, SD ;
MCEALL, E .
JOURNAL OF BACTERIOLOGY, 1970, 103 (03) :840-&
[7]  
DZCZESIUL M, 1976, BIOCHEMISTRY-US, V15, P2236
[8]  
FALCOZKELLY F, 1970, METHOD ENZYMOL, V17, P699
[10]   TARGET OF SERINE INHIBITION IN ESCHERICHIA-COLI [J].
HAMA, H ;
SUMITA, Y ;
KAKUTANI, Y ;
TSUDA, M ;
TSUCHIYA, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 168 (03) :1211-1216