THE VONKOSSA REACTION FOR CALCIUM DEPOSITS - SILVER LACTATE STAINING INCREASES SENSITIVITY AND REDUCES BACKGROUND

被引：85

作者：

RUNGBY, J ^{[1
]}

KASSEM, M ^{[1
]}

ERIKSEN, EF ^{[1
]}

DANSCHER, G ^{[1
]}

机构：

[1] AARHUS UNIV, STENO INST, DEPT NEUROBIOL, DK-8000 AARHUS, DENMARK

来源：

HISTOCHEMICAL JOURNAL | 1993年 / 25卷 / 06期

关键词：

D O I：

10.1007/BF00157809

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

The classical von Kossa method has been modified: the high silver nitrate concentration in the original was replaced by 0.05% silver lactate with hydroquinone remaining the reducing agent of choice. The present modification stained calcification nodules with a sensitivity comparable to the original von Kossa reaction, but resulted in a reduced background staining in cultured osteoblasts. The method works well also with plastic- or paraffin-embedded tissue sections.

引用

页码：446 / 451

页数：6

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