DEVELOPMENT OF NESTED PCR BASED ON THE VIAB SEQUENCE TO DETECT SALMONELLA-TYPHI

被引：54

作者：

HASHIMOTO, Y

ITHO, Y

FUJINAGA, Y

KHAN, AQ

SULTANA, F

MIYAKE, M

HIROSE, K

YAMAMOTO, H

EZAKI, T

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 03期

关键词：

D O I：

10.1128/JCM.33.3.775-777.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.

引用

页码：775 / 777

页数：3

共 3 条

[1] COMPLETE NUCLEOTIDE-SEQUENCE AND MOLECULAR CHARACTERIZATION OF VIAB REGION ENCODING VI ANTIGEN IN SALMONELLA-TYPHI [J].