CHARACTERIZATION OF THE THERMUS-THERMOPHILUS LOCUS ENCODING PEPTIDE DEFORMYLASE AND METHIONYL-TRNA(F)(MET) FORMYLTRANSFERASE

被引：100

作者：

MEINNEL, T

BLANQUET, S

机构：

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 23期

关键词：

D O I：

10.1128/JB.176.23.7387-7390.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An Escherichia coli strain with thermosensitive expression of the gene encoding peptide deformylase (fms) has been constructed. At nonpermissive temperatures, this strain fails to grow. The essential character of the fms gene was further used to clone by heterologous complementation the locus corresponding to Thermus thermophilus peptide deformylase. The cloned fragment also carries the methionyl-tRNA(f)(Met) formyltransferase gene (fmt). It is located immediately downstream from the fms gene, as in E. coli. Further sequence analysis of the region surrounding the E. coli fms-fmt locus indicates that the genes bordering the fms-fmt region are not conserved in T. thermophilus.

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页码：7387 / 7390

页数：4

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