INTERTRIBAL SOMATIC HYBRIDS BETWEEN BRASSICA-NAPUS AND BARBAREA-VULGARIS - PRODUCTION OF IN-VITRO PLANTLETS

Intertribal somatic hybrids were produced between Brassica napus and Barbarea vulgaris. The two species belong to the tribes Arabidae and Brassiceae, respectively. B. vulgaris is known to be cold tolerant and of interest to use as a gene donor to rapeseed. Of the plants produced in five fusion experiments six plants were to be hybrids by RFLP analysis one B. vulgaris-specific repetitive DNA sequence and two nuclear probes (rDNA and cruciferin) as markers. When analysing nuclear DNA content in four of these six hybrids, all had a higher DNA content than B. napus. However, mature plants could not be established outside in vitro indicating problems with between the species.