RAPID-DETERMINATION OF DOXORUBICIN AND ITS FLUORESCENT METABOLITES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：52

作者：

BAURAIN, R

DEPREZDECAMPENEERE, D

TROUET, A

机构：

[1] INT INST CELLULAR & MOLEC PATHOL, 74 AVE HIPPOCRATE, B-1200 BRUXELLES, BELGIUM

[2] CATHOLIC UNIV LOUVAIN, B-1200 BRUSSELS, BELGIUM

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90799-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An original method for the separation and quantitation of doxorubicin (DOX) [an antineoplastic drug] and its metabolites by high-pressure liquid chromatography [HPLC] and fluorometry is described. Doxorubicin and its derivatives are extracted from biological samples in a rapid, nondestructive manner, with a recovery close to 100%. The different compounds are rapidly separated by HPLC using an eluant system containing magnesium chloride, and detected quantitatively by fluorometry down to a concentration of 1.5 ng/ml in less than 5 min. Using this method, doxorubicin and its metabolites were determined in plasma and urine, after an i.v. injection into DBA2 and NMRI mice.

引用

页码：112 / 116

页数：5

共 11 条

[1]

BACHUR NR, 1974, J PHARMACOL EXP THER, V191, P331

[2] DETERMINATION OF DOXORUBICIN HYDROCHLORIDE IN PHARMACEUTICAL PREPARATIONS USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].