ESTROGENS, ANDROGENS, AND PROGESTINS IN FOLLICULAR-FLUID FROM PREOVULATORY FOLLICLES - IDENTIFICATION AND QUANTIFICATION BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY ASSOCIATED WITH STABLE ISOTOPE-DILUTION

被引:20
作者
DEHENNIN, L
机构
[1] Fondation de Recherche en Hormonologie, Fresnes
关键词
follicular fluid; gas chromatography/mass spectrometry; steroids;
D O I
10.1016/0039-128X(90)90108-N
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The synthesis and use of stable isotope-labeled analogs of various steroids have made it possible to undertake a study of follicular fluid (FF) aspirated from mature and preovulatory follicles. Our previous results have been brought together here in order to review quantitative work done by gas chromatography-mass spectrometry. A positive gradient between peripheral plasma and FF concentrations of a steroid suggests the possibility of ovarian biosynthesis. This is particularly relevant to the catecholestrogens, 19-norsteroids, and some corticosteroids. © 1992.
引用
收藏
页码:181 / 184
页数:4
相关论文
共 26 条
[1]  
Gore-Langton, Armstrong, Follicular steroidogenesis and its control, The Physiology of Reproduction, pp. 331-335, (1988)
[2]  
McNatty, Hunter, McNeilly, Sawers, Changes in the concentration of pituitary and steroid hormones in the follicular fluid of human Graafian follicles throughout the menstrual cycle, J Endocrinol, 64, pp. 555-571, (1975)
[3]  
Dehennin, Blacker, Reiffsteck, Scholler, Estrogen 2-, 4-, 6- or 16-hydroxylation by human ovarian follicles shown by gas chromatography-mass spectrometry associated with stable isotope dilution, J Steroid Biochem, 20, pp. 465-471, (1984)
[4]  
Silberzahn, Almahbobi, Dehennin, Merouane, Estrogen metabolites in equine ovarian follicles: gas chromatographic-mass spectrometric determinations in relation to follicular ultrastructure and progestin content, J Steroid Biochem, 22, pp. 501-505, (1985)
[5]  
Hammond, Hersey, Walega, Weisz, Catecholestrogen production by porcine ovarian cells, Endocrinology, 118, pp. 2292-2299, (1986)
[6]  
Mondschein, Hersey, Dey, Davis, Weisz, Catecholestrogen formation by pig blastocysts: biochemical characterization of estrogen-2/4-hydroxylase and correlation with aromatase activity during the preimplantation period, Endocrinology, 117, pp. 2339-2346, (1985)
[7]  
Hudson, Hillier, Catechol oestradiol control of FSH-stimulated granulosa cell steroidogenesis, J Endocrinol, 106, pp. R1-R4, (1985)
[8]  
Spicer, Hammond, Catecholestrogens stimulateprogestin secretion by cultured porcine granulosa cells, Mol Cell Endocrinol, 50, pp. 139-147, (1987)
[9]  
Spicer, Walega, Hammond, Metabolism of PH] 2-hydroxyestradiol by cultured porcine granulosa cells: evidence for the presence of a catechol-O-methyltransferase pathway and a direct stimulatory effect of 2-methoxyestradiol on progesterone production, Biol Reprod, 36, pp. 562-571, (1987)
[10]  
Dehennin, Jondet, Scholler, Androgen and 19norsteroid profiles in human preovulatory follicles from stimulated cycles: an isotope dilution-mass spectrometric study, J Steroid Biochem, 26, pp. 399-405, (1987)