PURIFICATION AND CHARACTERIZATION OF A MAJOR ALLERGEN OF ALTERNARIA-ALTERNATA

被引：35

作者：

DEARDS, MJ

MONTAGUE, AE

机构：

[1] Bencard Allergy Unit, Beecham Pharmaceuticals, Epsom, Surrey KT18 5XQ, Yew Tree Bottom Road

来源：

MOLECULAR IMMUNOLOGY | 1991年 / 28卷 / 4-5期

关键词：

D O I：

10.1016/0161-5890(91)90154-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A major allergen of Alternaria (Alt a Bd29K) was identified by SDS-PAGE and immunoblotting. It was purified by gel permeation and ion-exchange HPLC. The apparent molecular mass was 29,000, with a cysteine linked sub-unit of 15,000 M(r). The apparent isoelectric point was 4.2. It was found to be a constituent of the Alt I allergen group.

引用

页码：409 / 415

页数：7

共 17 条

[1]

AUKRUST L, 1982, Allergy (Copenhagen), V37, P36

[2] DETECTION OF ALLERGENS IN MOLD AND MITE PREPARATIONS BY A NITROCELLULOSE ELECTROBLOTTING TECHNIQUE [J].

BENGTSSON, A ;

KARLSSON, A ;

ROLFSEN, W ;

EINARSSON, R .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1986, 80 (04) :383-390

[3] ALLERGENS OF ALTERNARIA - A REVIEW OF RECENT CONCEPTS [J].

BUDD, TW .

GRANA, 1986, 25 (02) :147-154

[4] DETERMINATION OF PROTEIN - MODIFICATION OF LOWRY METHOD THAT GIVES A LINEAR PHOTOMETRIC RESPONSE [J].

HARTREE, EF .

ANALYTICAL BIOCHEMISTRY, 1972, 48 (02) :422-&

[5] PRODUCTION OF A PROPOSED INTERNATIONAL REFERENCE-STANDARD ALTERNARIA EXTRACT .2. RESULTS OF A COLLABORATIVE TRIAL [J].

HELM, RM ;

SQUILLACE, DL ;

YUNGINGER, JW .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1988, 81 (04) :651-663

[6]

HELM RM, 1989, FASEB J, V3, P1337

[7]

KROUTILL LA, 1980, J ALLERGY CLIN IMMUN, V80, P170

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] ALLERGEN NOMENCLATURE [J].

MARSH, DG ;

GOODFRIEND, L ;

KING, TP ;

LOWENSTEIN, H ;

PLATTSMILLS, TAE .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1987, 80 (05) :639-645

[10] SIMPLIFIED SILVER PROTEIN-DETECTION AND IMAGE-ENHANCEMENT METHODS IN POLYACRYLAMIDE GELS [J].

MERRIL, CR ;

GOLDMAN, D ;

VANKEUREN, ML .

ELECTROPHORESIS, 1982, 3 (01) :17-23

← 1 2 →