SPIN TRAPPING OF FREE-RADICALS AND LIPID-PEROXIDATION IN MICROSOMAL PREPARATIONS FROM MALIGNANT HYPERTHERMIA SUSCEPTIBLE PIGS

被引:15
作者
DUTHIE, GG
MCPHAIL, DB
ARTHUR, JR
GOODMAN, BA
MORRICE, PC
机构
[1] MACAULAY INST SOIL RES,ABERDEEN AB9 2QJ,SCOTLAND
[2] ROWETT RES INST,BUCKSBURN AB2 9SB,ABERDEEN,SCOTLAND
来源
FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 8卷 / 02期
关键词
Antioxidant abnormality; Electron spin resonance; Free radicals; Lipid peroxidation; Malignant hyperthermia;
D O I
10.3109/10715769009087979
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microsomes were prepared from livers of malignant hyperthermia susceptible (MHS) or resistant (MHR) pigs. On incubation with the spin trap α4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN), the microsomes from MHS pigs produced a characteristic electron spin resonance (ESR) signal at a greater rate than those from MHR pigs. Increased formation in the incubations of thiobarbituric acid reactive substances (TBARS) by the microsomes of the MHS pigs indicated an enhanced susceptibility to free radical-mediated lipid peroxidation. These results provide further evidence that MHS pigs have an antioxidant abnormality which may contribute to the fatal MH response. However the nature of the abnormality is unclear. The enhanced formation of unstable free radicals and indices of lipid peroxidation was not due to decreased vitamin E concentration or glutathione peroxidase activity in the microsomes. Furthermore, fatty acid profiles were similar in microsomes from MHS and MHR pigs indicating similar amounts of potential substrate for TBARS formation © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
引用
收藏
页码:93 / 99
页数:7
相关论文
共 22 条
[2]   SPIN TRAPPING OF FREE-RADICALS IN HOMOGENATES OF HEART FROM SELENIUM AND VITAMIN-E DEFICIENT RATS [J].
ARTHUR, JR ;
MCPHAIL, DB ;
GOODMAN, BA .
FREE RADICAL RESEARCH COMMUNICATIONS, 1988, 4 (05) :311-315
[3]  
BRAUGHLER JM, 1988, OXYGEN RADICALS TISS, P99
[4]   MALIGNANT HYPERTHERMIA [J].
BRITT, BA .
CANADIAN ANAESTHETISTS SOCIETY JOURNAL, 1985, 32 (06) :666-677
[5]   SPIN TRAPPING - ELECTRON-SPIN-RESONANCE PARAMETERS OF SPIN ADDUCTS [J].
BUETTNER, GR .
FREE RADICAL BIOLOGY AND MEDICINE, 1987, 3 (04) :259-303
[6]   A SEARCH FOR OXYGEN-CENTERED FREE-RADICALS IN THE LIPOXYGENASE LINOLEIC-ACID SYSTEM [J].
CONNOR, HD ;
FISCHER, V ;
MASON, RP .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 141 (02) :614-621
[7]   INCREASED INDEXES OF LIPID-PEROXIDATION IN STRESS-SUSCEPTIBLE PIGS AND EFFECTS OF VITAMIN-E [J].
DUTHIE, GG ;
ARTHUR, JR ;
NICOL, F ;
WALKER, M .
RESEARCH IN VETERINARY SCIENCE, 1989, 46 (02) :226-230
[8]  
DUTHIE GG, 1987, AM J VET RES, V48, P309
[9]  
DUTHIE GG, 1989, AM J VET RES, V50, P84
[10]  
DUTHIE GG, 1989, IN PRESS ANN NY ACAD