FERMENTATION OF CANDIDA-UTILIS FOR URICASE PRODUCTION

被引：14

作者：

ADAMEK, V

SUCHOVA, M

DEMNEROVA, K

KRALOVA, B

FORT, I

MORAVA, P

机构：

[1] Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague

[2] Department of Chemical and Food Process Equipment Design, Czech Technical University, Prague

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 6卷 / 02期

关键词：

Candida utilis; Fermentation kinetics; Inducer of uricase production; Uricase production;

D O I：

10.1007/BF01576427

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conditions for the production of microbial uricase by Candida utilis were studied. For the selected strain, hypoxanthine proved to be the most effective inducer of uricase formation. The highest values of biomass as well as uricase activity in the mechanically agitated fermentor were obtained under the following conditions: 50 h, rotation impeller speed 7 s-1, air flow rate 1.25×10-5 m3s-1, concentration of inducer 0.1%. © 1990 Society for Industrial Microbiology.

引用

页码：85 / 89

页数：5

共 7 条

[1]

Bongaerts G.P.A., Uitzetter J., Brouns R., Vogels G.D., Uricase of Bacillus fastidiosus properties and regulation of synthesis, Biochim. Biophys. Acta, 527, pp. 348-358, (1978)

[2]

Itaya K., Yamamoto T., Fukumoto J., Studies on yeast uricase, Agricultural and Biological Chemistry, 31, pp. 1256-1264, (1967)

[3]

Itaya K., Yamamoto T., Fukumoto J., Studies on urate oxidase of Candida utilis, Agricultural and Biological Chemistry, 35, pp. 813-821, (1971)

[4]

Kralova B., Lehejckova R., Demnerova K., Dobransky T., Extraction of uricase from Candida utilis, Biotechnol. Lett., 8, pp. 99-10, (1986)

[5]

Lowry O.H., Roseborough N.J., Farr A.L., Randall R.J., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, pp. 265-275, (1951)

[6]

Mahler J.L., A new bacterial uricase for uric acid determination, Anal. Biochem., 38, pp. 65-68, (1970)

[7]

Nishimura M., Yoshida K., Yokota Y., Matsushida A., Mada Y., Physiochemical properties and states of sulphydryl groups of uricase from Candida utilis, J. Biochem., 91, pp. 41-48, (1982)

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