PREPARATIVE HIGH-PERFORMANCE LIQUID PARTITION CHROMATOGRAPHY OF PROTEINS

被引：99

作者：

RUBINSTEIN, M

机构：

[1] Roche Institute of Molecular Biology, Nutley

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90697-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for preparative high-performance liquid chromatography (hplc) of proteins are described. Both normal and reverse-phase chromatography were studied and adapted to the fractionation of proteins in quantities of up to 50 mg. Lichrosorb Diol was used as a normal phase" for chromatography of hydrophobic proteins. Lichrosorb RP-8 was used for reversephase chromatography of proteins. © 1979."

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页码：1 / 7

页数：7

相关论文

共 21 条

[1] AUTOMATIC MONITORING OF PRIMARY AMINES IN PREPARATIVE COLUMN EFFLUENTS WITH FLUORESCAMINE [J].

BOHLEN, P ;

STEIN, S ;

STONE, J ;

UDENFRIEND, S .

ANALYTICAL BIOCHEMISTRY, 1975, 67 (02) :438-445

[2]

Burgus R, 1976, PEPTIDES 1976, P85

[3]

CHANG SH, 1976, J CHROMATOGR, V125, P103, DOI 10.1016/S0021-9673(00)93814-7

[4] REVERSED-PHASE, HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF PEPTIDES AND PROTEINS WITH ION-PAIRING REAGENTS [J].

HANCOCK, WS ;

BISHOP, CA ;

PRESTIDGE, RL ;

HARDING, DRK ;

HEARN, MTW .

SCIENCE, 1978, 200 (4346) :1168-1170

[5] PURIFICATION TO HOMOGENEITY OF CAMEL PITUITARY PRO-OPIOCORTIN, THE COMMON PRECURSOR OF OPIOID PEPTIDES AND CORTICOTROPIN [J].

KIMURA, S ;

LEWIS, RV ;

GERBER, LD ;

BRINK, L ;

RUBINSTEIN, M ;

STEIN, S ;

UDENFRIEND, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (04) :1756-1759

[6] COMMON PRECURSOR TO CORTICOTROPINS AND ENDORPHINS [J].

MAINS, RE ;

EIPPER, BA ;

LING, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (07) :3014-3018

[7] SEPARATION OF AMINO-ACIDS AND PEPTIDES ON NONPOLAR STATIONARY PHASES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

MOLNAR, I ;

HORVATH, C .

JOURNAL OF CHROMATOGRAPHY, 1977, 142 (NOV) :623-640

[8]

MONCH W, 1978, J CHROMATOGR, V147, P415, DOI 10.1016/S0021-9673(00)85159-6

[9] PURIFICATION OF GROWTH-PROMOTING PEPTIDES AND PROTEINS, AND OF HISTONES, BY HIGH-PRESSURE SILICA-GEL CHROMATOGRAPHY [J].

PICKART, LR ;

THALER, MM .

PREPARATIVE BIOCHEMISTRY, 1975, 5 (5-6) :397-412

[10] HIGH-EFFICIENCY CATION-EXCHANGE CHROMATOGRAPHY OF POLYPEPTIDES AND POLYAMINES IN NANOMOLE RANGE [J].

RADHAKRISHNAN, AN ;

STEIN, S ;

LICHT, A ;

GRUBER, KA ;

UDENFRIEND, S .

JOURNAL OF CHROMATOGRAPHY, 1977, 132 (03) :552-555