EXTRACTION AND CHARACTERIZATION OF MESSENGER-RNA FROM MYCOBACTERIA - IMPLICATION FOR VIRULENCE GENE IDENTIFICATION

被引：20

作者：

PATEL, BKR ^{[1
]}

BANERJEE, DK ^{[1
]}

BUTCHER, PD ^{[1
]}

机构：

[1] ST GEORGE HOSP, SCH MED, DEPT MED MICROBIOL, LONDON, ENGLAND

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1991年 / 13卷 / 02期

关键词：

CDNA; HEAT-SHOCK; MYCOBACTERIUM; PCR; MESSENGER RNA;

D O I：

10.1016/0167-7012(91)90010-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the extraction of intact total RNA from mycobacteria (Mycobacterium bovis BCG and M. leprae) has been developed. The presence of specific mRNA transcripts within this population has been confirmed by the following evidence: hybridization of Northern blots with gene-specific probes revealed discrete size classes of transcript; increases in mRNA levels for the 71kDa heat-shock protein were seen after heat shock and this was abolished with rifampicin; cDNA specific for hsp-71kDa mRNA could be synthesized from total RNA and characterized after PCR amplification. The ability to analyse mRNA by this method has implications for the study of gene expression and the molecular mechanisms of pathogenicity in mycobacteria.

引用

页码：99 / 111

页数：13

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