EXPRESSION OF THE BARLEY PSBA GENE IN ESCHERICHIA-COLI YIELDS A FUNCTIONAL IN-VITRO PHOTOSYSTEM-II PROTEIN D1

被引：7

作者：

EFIMOV, VA ^{[1
]}

FRADKOV, AF ^{[1
]}

RASKIND, AB ^{[1
]}

KHRISTIN, MS ^{[1
]}

KLIMOV, VV ^{[1
]}

CHAKHMAKHCHEVA, OG ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST SOIL SCI & PHOTOSYNTH,PUSHCHINO 142292,RUSSIA

来源：

FEBS LETTERS | 1994年 / 348卷 / 02期

关键词：

PHOTOSYSTEM II; RECOMBINANT D1 PROTEIN; HISTIDINE-BINDING DOMAIN; PROTEIN-PIGMENT COMPLEX;

D O I：

10.1016/0014-5793(94)00586-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The barley chloroplast psbA gene encoding D1 protein, one of the main photosystem II components, has been over-expressed in E. coli cells. The existance of two in vivo expression products, a protein with Mr about 33.5 kDa, corresponding to the full-length precursor of the 32 kDa D1 mature form, and a truncated 29 kDa polypeptide was revealed. A modified D1 protein containing six histidine residues at the carboxy-terminus was also obtained. After isolation and renaturation, the ability of the recombinant D1 protein to bind atrazine and pigments from barley thylakoids was demonstrated.

引用

页码：153 / 157

页数：5

共 16 条

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