REPEATED INTRAPORTAL HEPATOCYTE TRANSPLANTATION IN ANALBUMINEMIC RATS

被引：70

作者：

ROZGA, J ^{[1
]}

HOLZMAN, M ^{[1
]}

MOSCIONI, AD ^{[1
]}

FUJIOKA, H ^{[1
]}

MORSIANI, E ^{[1
]}

DEMETRIOU, AA ^{[1
]}

机构：

[1] CEDARS SINAI MED CTR,LIVER SUPPORT UNIT,LOS ANGELES,CA 90048

来源：

CELL TRANSPLANTATION | 1995年 / 4卷 / 02期

关键词：

HEPATOCYTE; LIVER; TRANSPLANTATION; RAT;

D O I：

10.1016/0963-6897(95)90035-7

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The optimal site for implantation of isolated hepatocytes has not been established. We have developed a novel technique which allows repeated infusion of hepatocytes into the portal system via an indwelling catheter. Seven Nagase Analbuminemic rats (NAR) underwent single intraportal infusion of 2 x 10(7) isolated normal albumin-producing rat hepatocytes. Another seven NAR rats underwent placement of indwelling catheters into the portal venous system via the gastroduodenal vein, Each of them received six batches of 5 x 10(6) normal albumin producing hepatocytes. Seven control NAR rats were infused repeatedly (intraportally) with saline only. Plasma albumin (ELISA) showed significant increase in experimental animals and was more pronounced (p < 0.05) in rats transplanted repeatedly than in those given a single dose of cells. Immunohistochemical staining of the liver sections confirmed the presence of transplanted albumin producing hepatocytes. Rats transplanted with a single large batch of isolated hepatocytes showed liver tissue damage, whereas those subjected to repeated cell infusions had normal liver histology. We have developed a novel intraportal transplantation method which allows successful engraftment of a large number of isolated hepatocytes.

引用

页码：237 / 243

页数：7

共 13 条

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