CLONING OF CDNA-ENCODING THE BIFUNCTIONAL DEHYDROQUINASE-CENTER-DOT-SHIKIMATE DEHYDROGENASE OF AROMATIC-AMINO-ACID BIOSYNTHESIS IN NICOTIANA-TABACUM

被引：30

作者：

BONNER, CA

JENSEN, RA

机构：

[1] Department Microbiology Cell Science, 3103 McCarty Hall, University of Florida, Gainsville

来源：

BIOCHEMICAL JOURNAL | 1994年 / 302卷

关键词：

D O I：

10.1042/bj3020011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nicotiana tabacum cDNA encoding a bifunctional protein having catalytic domains for dehydroquinase and shikimate dehydrogenase was cloned and sequenced. Complementation of Escherichia coli aroD and aroE auxotrophs was successful. Amino acid sequencing located the N-terminus of the mature protein. The two catalytic domains exhibited greater amino acid identity with prokaryote homologues than with yeast and fungal homologues.

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页码：11 / 14

页数：4

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