BIDIRECTIONAL ISOTACHOPHORESIS .2. 15 ELECTROLYTE SYSTEMS COVERING THE PH-RANGE-3.5-10

被引:13
作者
HIROKAWA, T
机构
[1] Applied Physics and Chemistry, faculty of Engineering, Hiroshima University, Higashi-Hiroshima, 724
关键词
D O I
10.1016/S0021-9673(94)89016-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fifteen electrolyte systems useful for bidirectional isotachophoresis were proposed covering the pH range 3.5-10. The pH dependence on the R(E) values of the terminating zones was simulated for all the electrolyte systems proposed, together with the isotachophoretic velocities. A 10 mM HCl solution was the anionic leading electrolyte and a 10 mM KOH solution the cationic one, of which the pH was adjusted by adding ten kinds of pH buffers. The present simulation might be conveniently used to select an appropriate bidirectional electrolyte system for the samples of interest.
引用
收藏
页码:158 / 163
页数:6
相关论文
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HIROKAWA, T ;
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JOURNAL OF CHROMATOGRAPHY, 1993, 633 (1-2) :251-259