DETECTION OF ASTROVIRUSES FROM STOOL SAMPLES IN JAPAN USING REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION AMPLIFICATION

被引：32

作者：

SAITO, K

USHIJIMA, H

NISHIO, O

OSETO, MK

MOTOHIRO, H

UEDA, Y

TAKAGI, M

NAKAYA, S

ANDO, T

GLASS, R

ZAIMAN, K

机构：

[1] INST PUBL HLTH, DEPT MICROBIOL, MINATO KU, TOKYO 108, JAPAN

[2] TOKYO UNIV AGR, DEPT CLIN NUTR, SETAGAYA KU, TOKYO 156, JAPAN

[3] EHIME PREFECTURAL INST PUBL HLTH, DIV VIRUS, MATSUYAMA, EHIME 790, JAPAN

[4] KURUME UNIV, SCH MED, DEPT PEDIAT, KURUME, FUKUOKA 830, JAPAN

[5] MAIZURU KYOSAI HOSP, DEPT PEDIAT, MAIZURU, KYOTO 625, JAPAN

[6] CTR DIS CONTROL & PREVENT, VIRAL GASTROENTERITIS SECT, ATLANTA, GA 30333 USA

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1995年 / 39卷 / 10期

关键词：

ASTROVIRUS; RT-PCR; ENZYME IMMUNOASSAY; EPIDEMIOLOGY;

D O I：

10.1111/j.1348-0421.1995.tb03264.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We developed a reverse transcription and polymerase chain reaction (RT-PCR) method for detecting astrovirus serotypes 1, 2, 3, 5, 6 and 7 (but not serotype 4). Furthermore, we developed the specific primers for detecting serotypes 1 and 2, the most predominant serotypes in the world. Sensitivity of the first PCR with serotype common primers was about 10 times higher than that of enzyme immunoassay with monoclonal antibody (EIA-MAb). Sensitivity of the second PCR with the serotype-specific primers was even higher. The RT-PCR method was useful for detecting astroviruses from clinical samples, especially serotypes 1 and 2.

引用

页码：825 / 828

页数：4

共 17 条

[1] IDENTIFICATION OF GROUP-A ROTAVIRUS GENE-4 TYPES BY POLYMERASE CHAIN-REACTION [J].