ENHANCEMENT OF FELINE INFECTIOUS PERITONITIS VIRUS TYPE-I INFECTION IN CELL-CULTURES USING LOW-SPEED CENTRIFUGATION

被引：5

作者：

HOHDATSU, T

TATEKAWA, T

KOYAMA, H

机构：

[1] Department of Veterinary Infectious Diseases, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 51卷 / 2-3期

关键词：

FELINE INFECTIOUS PERITONITIS VIRUS; CENTRIFUGAL INOCULATION; ENHANCEMENT;

D O I：

10.1016/0166-0934(94)00119-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of centrifugation on the ability of feline infectious peritonitis virus (FIPV) to infect cells in culture was investigated. The infectivity titer was the highest when the plates were centrifuged at 400 X g (1500 rpm) for 2 h. All five strains classified as FIPV Type I showed infectivity titers enhanced 10-100-fold by centrifugation at 400 X g for 2 h. The centrifugal enhancement of infection was obtained only by centrifugation immediately after inoculation of the virus, suggesting that the enhancement occurs during attachment or adsorption of viruses to the cells. This method may be useful for the culture of FIPV Type I strains.

引用

页码：357 / 362

页数：6

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