FLUORESCENCE IN THE TANDEM SCANNING MICROSCOPE

被引:31
作者
BOYDE, A
JONES, SJ
TAYLOR, ML
WOLFE, LA
WATSON, TF
机构
[1] Department of Anatomy and Developmental Biology, University College London, London
关键词
bone; bone cells; Confocal; fluorescence; restorations; tandem scanning; teeth;
D O I
10.1111/j.1365-2818.1990.tb02945.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
Our studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real‐time viewing and real colour, which allow faster use and interpretation. CSLM are complementary, not competitive, being currently more sophisticated for low‐level fluorescence work. This is equally possible with available TSM, but requires further development using CCD cameras and image‐processing systems. 1990 Blackwell Science Ltd
引用
收藏
页码:39 / 49
页数:11
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