OBSERVATIONS REGARDING DNA-REPLICATION SITES IN HUMAN-CELLS INVIVO FOLLOWING INFUSIONS OF IODODEOXYURIDINE AND BROMODEOXYURIDINE

被引：15

作者：

RAZA, A

MILLER, MA

MAZEWSKI, C

SHEIKH, Y

LAMPKIN, B

SAWAYA, R

CRONE, K

BERGER, T

REISING, J

GRAY, J

KHAN, S

PREISLER, H

机构：

[1] UNIV CINCINNATI, MED CTR, CHILDRENS HOSP, CINCINNATI, OH 45267 USA

[2] UNIV CINCINNATI, MED CTR, DEPT NEUROSURG, CINCINNATI, OH 45267 USA

[3] UNIV CINCINNATI, MED CTR, DEPT THORAC SURG, CINCINNATI, OH 45267 USA

[4] NEW YORK STATE DEPT HLTH, ROSWELL PK MEM INST, BUFFALO, NY 14263 USA

[5] UNIV CALIF LAWRENCE LIVERMORE NATL LAB, LIVERMORE, CA 94550 USA

来源：

CELL PROLIFERATION | 1991年 / 24卷 / 02期

关键词：

D O I：

10.1111/j.1365-2184.1991.tb01143.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In studies using bromodeoxyuridine (BrdUrd) and/or iododeoxyuridine (IdUrd) to label S phase cells in cancer patients, several unique observations were made regarding DNA replication sites and the organization of newly synthesized DNA in post-mitotic cells. While the majority of tumour specimens removed at the end of infusions demonstrated concentration of replication sites around the nuclear membrane, biopsies obtained in leukaemic patients 1 week later demonstrated several distinct patterns of labelling. For example, one, two or all lobes of granulocytes were labelled. Scavenger macrophages bearing labelled leukaemic cells in their cytoplasm were also seen. Sequential IdUrd/BrdUrd labelling of solid tumours showed various patterns of nuclear/nucleolar/membrane labelling, allowing more precise localization of early versus late replication sites.

引用

页码：113 / 126

页数：14

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