AUTOMATED PROCEDURE FOR DEWAXING AND REHYDRATION OF PARAFFIN-EMBEDDED TISSUE-SECTIONS FOR DNA FLOW CYTOMETRIC ANALYSIS OF BREAST-TUMORS

被引：7

作者：

BABIAK, J

POPPEMA, S

机构：

[1] Department of Laboratory Medic, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 96卷 / 01期

关键词：

FLOW CYTOMETRY; PARAFFIN-EMBEDDED TISSUE; TISSUE PROCESSING; DNA ANALYSIS;

D O I：

10.1093/ajcp/96.1.64

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Flow cytometric DNA analysis of paraffin-embedded tumors is an important diagnostic and prognostic tool in clinical pathology. The technique is limited, however, by the time-consuming multistep procedure for dewaxing and rehydrating tissue. The authors developed an automated procedure to complete the dewaxing and rehydration of tissue using a routine histologic tissue processor with a 24-hour timer. This technique provided excellent tissue recovery and reproducible DNA histograms comparable to those obtained by manual methods. Subsequently, the authors analyzed the DNA content of 93 paraffin-embedded breast cancer tissues. The automation of a significant portion of the routine processing required for paraffin-embedded tissue makes cytometric DNA analysis a more practical procedure in the laboratory.

引用

页码：64 / 69

页数：6

共 13 条

[1] APPLICATION OF DNA FLOW-CYTOMETRY TO PARAFFIN-EMBEDDED ARCHIVAL MATERIAL FOR THE STUDY OF ANEUPLOIDY AND ITS CLINICAL-SIGNIFICANCE [J].

HEDLEY, DW ;

FRIEDLANDER, ML ;

TAYLOR, IW .

CYTOMETRY, 1985, 6 (04) :327-333

[2] METHOD FOR ANALYSIS OF CELLULAR DNA CONTENT OF PARAFFIN-EMBEDDED PATHOLOGICAL MATERIAL USING FLOW-CYTOMETRY [J].

HEDLEY, DW ;

FRIEDLANDER, ML ;

TAYLOR, IW ;

RUGG, CA ;

MUSGROVE, EA .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1983, 31 (11) :1333-1335

[3]

HEDLEY DW, 1984, CANCER RES, V44, P5395

[4] CONVENTION ON NOMENCLATURE FOR DNA CYTOMETRY [J].

HIDDEMANN, W ;

SCHUMANN, J ;

ANDREEFF, M ;

BARLOGIE, B ;

HERMAN, CJ ;

LEIF, RC ;

MAYALL, BH ;

MURPHY, RF ;

SANDBERG, AA .

CYTOMETRY, 1984, 5 (05) :445-446

[5] TUMOR DNA PLOIDY AS AN INDEPENDENT PROGNOSTIC FACTOR IN BREAST-CANCER [J].

KALLIONIEMI, OP ;

BLANCO, G ;

ALAVAIKKO, M ;

HIETANEN, T ;

MATTILA, J ;

LAUSLAHTI, K ;

KOIVULA, T .

BRITISH JOURNAL OF CANCER, 1987, 56 (05) :637-642

[6]

KALLIONIEMI OP, 1988, CANCER, V62, P2183, DOI 10.1002/1097-0142(19881115)62:10<2183::AID-CNCR2820621019>3.0.CO

[7]

2-B

[8]

MUSS HB, 1989, CANCER, V64, P1894, DOI 10.1002/1097-0142(19891101)64:9<1894::AID-CNCR2820640923>3.0.CO

[9]

2-K

[10] TUMOR ANEUPLOIDY, PROGNOSTIC PARAMETERS AND SURVIVAL IN PRIMARY BREAST-CANCER [J].

OWAINATI, AAR ;

ROBINS, RA ;

HINTON, C ;

ELLIS, IO ;

DOWLE, CS ;

FERRY, B ;

ELSTON, CW ;

BLAMEY, RW ;

BALDWIN, RW .

BRITISH JOURNAL OF CANCER, 1987, 55 (04) :449-454

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