SIMULTANEOUS DETERMINATION OF COTININE AND TRANS-3'-HYDROXYCOTININE IN HUMAN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：22

作者：

PICHINI, S

ALTIERI, I

PACIFICI, R

ROSA, M

OTTAVIANI, G

ZUCCARO, P

机构：

[1] IST SUPER SANITA,DEPT CLIN BIOCHEM,VIALE REGINA ELENA 299,I-00161 ROME,ITALY

[2] AERONAUT MIL,IST MED LEGALE ALDO LORETO,ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 577卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80259-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method with ultraviolet photometric detection has been developed for the quantitation of cotinine and trans-3'-hydroxycotinine in human serum. A solid-phase extraction procedure was performed for the analytes and the internal standard. N-ethylnorcotinine, before chromatography. The use of a 30-cm reversed-phase column and a mobile phase of water-methanol-0.1 M sodium acetate-acetonitrile (67:24.5:6.5:2, v/v), pH 4.3, prevented the co-elution of caffeine with cotinine. The limit of quantitation observed with this method was 5 ng/ml for both cotinine and trans-3'-hydroxycotinine. The present method proved useful for the determination of serum levels of these metabolites, correlating with nicotine daily intake.

引用

页码：358 / 361

页数：4

共 18 条

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