ANALYSIS OF URINARY ORGANIC-ACIDS BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY

被引：10

作者：

KAJITA, M ^{[1
]}

NIWA, T ^{[1
]}

WATANABE, K ^{[1
]}

机构：

[1] NAGOYA UNIV,BRANCH HOSP,DEPT INTERNAL MED,HIGASHI KU,NAGOYA 461,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 622卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80275-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We developed a new method for the rapid determination of urinary organic acids using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. Mass spectra of authentic organic acids obtained in the negative-ion mode showed intense [M-H](-) ions with some fragment ions. Urine samples of patients with methylmalonic aciduria, ornithine transcarbamylase deficiency, and phenylketonuria were extracted using anion-exchange columns. The mass chromatograms of the extracts showed some dominant peaks of abnormal metabolites characteristic of each disorder. This is a useful method for the analysis of urinary organic acids for the diagnosis of organic aciduria, because the sample preparation is simple.

引用

页码：263 / 268

页数：6

共 12 条

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