PEPTIDE SEQUENCING IDENTIFIES MSS1, A MODULATOR OF HIV TAT-MEDIATED TRANSACTIVATION, AS SUBUNIT-7 OF THE 26-S PROTEASE

被引：86

作者：

DUBIEL, W ^{[1
]}

FERRELL, K ^{[1
]}

RECHSTEINER, M ^{[1
]}

机构：

[1] UNIV UTAH,SCH MED,DEPT BIOCHEM,SALT LAKE CITY,UT 84112

来源：

FEBS LETTERS | 1993年 / 323卷 / 03期

关键词：

MSS1; HIV; HUMAN-26; S-PROTEASE; PUTATIVE ATPASE; TAT;

D O I：

10.1016/0014-5793(93)81356-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Subunit 7 is an integral component of the human erythrocyte 26 S protease. Peptide sequence analysis reveals that 22 amino acids from the N-terminus of subunit 7 correspond exactly to the N-terminus of MSS1, a modulator of HIV gene expression. Additional internal peptides from subunit 7 obtained by CNBr cleavage also match 100% with the deduced amino acid sequence of MSS1. Based on the fact that directly sequenced peptides from subunit 7 are identical to more than 12% of the hypothetical translation product of MSS1, and the fact that the molecular weight of subunit 7 (49 kDa) corresponds to the predicted molecular weight of MSS1 (48,633 Da), we conclude that subunit 7 is MSS1.

引用

页码：276 / 278

页数：3

共 23 条

[1] CHANGES IN INTRACELLULAR-LOCALIZATION OF PROTEASOMES IN IMMORTALIZED OVARIAN GRANULOSA-CELLS DURING MITOSIS ASSOCIATED WITH A ROLE IN CELL-CYCLE CONTROL [J].

AMSTERDAM, A ;

PITZER, F ;

BAUMEISTER, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (01) :99-103

[2]

DRISCOLL J, 1990, J BIOL CHEM, V265, P4789

[3]

DUBIEL W, 1992, J BIOL CHEM, V267, P22699

[4] PAS1, A YEAST GENE REQUIRED FOR PEROXISOME BIOGENESIS, ENCODES A MEMBER OF A NOVEL FAMILY OF PUTATIVE ATPASES [J].

ERDMANN, R ;

WIEBEL, FF ;

FLESSAU, A ;

RYTKA, J ;

BEYER, A ;

FROHLICH, KU ;

KUNAU, WH .

CELL, 1991, 64 (03) :499-510

[5] ATP-DEPENDENT INCORPORATION OF 20S PROTEASE INTO THE 26S COMPLEX THAT DEGRADES PROTEINS CONJUGATED TO UBIQUITIN - (PROTEIN BREAKDOWN MULTICATALYTIC PROTEINASE COMPLEX) [J].

EYTAN, E ;

GANOTH, D ;

ARMON, T ;

HERSHKO, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) :7751-7755

[6] YEAST-CELL CYCLE PROTEIN CDC48P SHOWS FULL-LENGTH HOMOLOGY TO THE MAMMALIAN PROTEIN VCP AND IS A MEMBER OF A PROTEIN FAMILY INVOLVED IN SECRETION, PEROXISOME FORMATION, AND GENE-EXPRESSION [J].

FROHLICH, KU ;

FRIES, HW ;

RUDIGER, M ;

ERDMANN, R ;

BOTSTEIN, D ;

MECKE, D .

JOURNAL OF CELL BIOLOGY, 1991, 114 (03) :443-453

[7]

GANOTH D, 1988, J BIOL CHEM, V263, P12412

[8] ISOLATION OF A YEAST GENE ENCODING A PROTEIN HOMOLOGOUS TO THE HUMAN TAT-BINDING PROTEIN TBP-1 [J].

GOYER, C ;

LEE, HS ;

MALO, D ;

SONENBERG, N .

DNA AND CELL BIOLOGY, 1992, 11 (08) :579-585

[9]

HOFFMAN L, 1992, J BIOL CHEM, V267, P22362

[10]

HOUGH R, 1986, J BIOL CHEM, V261, P2400

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