Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures

被引：157

作者：

Bronstein, DM ^{[1
]}

PerezOtano, I ^{[1
]}

Sun, V ^{[1
]}

Sawin, SBM ^{[1
]}

Chan, J ^{[1
]}

Wu, GC ^{[1
]}

Hudson, PM ^{[1
]}

Kong, LY ^{[1
]}

Hong, JS ^{[1
]}

McMillian, MK ^{[1
]}

机构：

[1] NIEHS,ENVIRONM NEUROSCI LAB,RES TRIANGLE PK,NC 27709

来源：

BRAIN RESEARCH | 1995年 / 704卷 / 01期

关键词：

lipopolysaccharide; astrocyte; microglia; 6-hydroxydopamine; tyrosine hydroxylase; dopamine; Parkinson's disease; Substantia nigra;

D O I：

10.1016/0006-8993(95)01189-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly-L-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% Of the tyrosine hydroxylase (TH)-immunopositive neurons, but LPS was not neurotoxic. Conversely, in mixed neuron/glial cultures, 6-OHDA killed only 27% of the TH-immunopositive neurons while LPS killed 70%. The mixed neuronal/glial mesencephalic culture offers a better in vitro model for studying possible mechanisms involved in Parkinson's disease.

引用

页码：112 / 116

页数：5

共 40 条

[1] MICROGLIAL RESPONSE TO 6-HYDROXYDOPAMINE-INDUCED SUBSTANTIA NIGRA LESIONS
AKIYAMA, H
MCGEER, PL
[J]. BRAIN RESEARCH, 1989, 489 (02) : 247 - 253
[2] TROPHIC ACTIONS OF TRANSFORMING GROWTH-FACTOR-ALPHA ON MESENCEPHALIC DOPAMINERGIC-NEURONS DEVELOPING IN CULTURE
ALEXI, T
HEFTI, F
[J]. NEUROSCIENCE, 1993, 55 (04) : 903 - 918
[3] ASCHNER M, 1991, NEUROTOXICOLOGY, V12, P505
[4] CYTOTOXICITY OF MICROGLIA
BANATI, RB
GEHRMANN, J
SCHUBERT, P
KREUTZBERG, GW
[J]. GLIA, 1993, 7 (01) : 111 - 118
[5] CELLS FROM EMBRYONIC RAT STRIATUM COCULTURED WITH MESENCEPHALIC GLIA EXPRESS DOPAMINERGIC PHENOTYPES
BEYER, C
PILGRIM, C
REISERT, I
MISGELD, U
[J]. NEUROSCIENCE LETTERS, 1991, 128 (01) : 1 - 3
[6] ACTIVE UPTAKE OF MPP+, A METABOLITE OF MPTP, BY BRAIN SYNAPTOSOMES
CHIBA, K
TREVOR, AJ
CASTAGNOLI, N
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 128 (03) : 1228 - 1232
[7] DIMONTE DA, 1992, ANN NY ACAD SCI, V648, P219
[8] CELL-DAMAGE ASSOCIATED WITH CHANGING THE MEDIUM OF MESENCEPHALIC CULTURES IN SERUM-FREE MEDIUM IS MEDIATED VIA N-METHYL-D-ASPARTATE RECEPTORS
DRISCOLL, BF
LAW, MJ
CRANE, AM
[J]. JOURNAL OF NEUROCHEMISTRY, 1991, 56 (04) : 1201 - 1206
[9] ENGELE J, 1991, J NEUROSCI, V11, P3070
[10] CORTICAL ASTROCYTES ACTIVATED BY BASIC FIBROBLAST GROWTH-FACTOR SECRETE MOLECULES THAT STIMULATE DIFFERENTIATION OF MESENCEPHALIC DOPAMINERGIC-NEURONS
GAUL, G
LUBBERT, H
[J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1992, 249 (1324) : 57 - 63

← 1 2 3 4 →