FOLATE UPTAKE IN L1210 CELLS - MEDIATION BY AN ADENINE TRANSPORT-SYSTEM

被引：21

作者：

SURESH, MR

HENDERSON, GB

HUENNEKENS, FM

机构：

[1] Department of Biochemistry Scripps Clinic and Research Foundation La Jolla

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(79)91657-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uptake of folate by L1210 cells in mediated by a transport system whose primary substrate is adenine. This conclusion is based upon the following evidence: (a) Folate uptake is inhibited competitively by adenine; (b) The Kt for folate transport (430 μM) is comparable to the Ki (450 μM) for folate inhibition of adenine transport; (c) The Kt for adenine transport (21 μM) agrees with the Ki (17 μM) for inhibition of folate transport by adenine; (d) The adenine analogs, 1-methyl-3-isobutylxanthine and 6-mercapto-purine, each inhibit folate and adenine transport to a comparable degree; and (e) Rates of folate and adenine uptake vary in parallel fashion during growth of L1210 cells. © 1979.

引用

页码：135 / 139

页数：5

共 9 条

[1] NATURALLY OCCURRING FOLATES IN BLOOD AND LIVER OF RAT [J].

BIRD, OD ;

MCGLOHON, VM ;

VAITKUS, JW .

ANALYTICAL BIOCHEMISTRY, 1965, 12 (01) :18-&

[2]

GOLDMAN ID, 1971, ANN NY ACAD SCI, V186, P400

[3]

GORNALL AG, 1949, J BIOL CHEM, V177, P751

[4]

HENDERSON GB, 1978, CANCER RES, V38, P859

[5] STUDIES ON IDENTIFICATION OF A FOLATE COMPOUND OF HUMAN SERUM [J].

HERBERT, V ;

LARRABEE, AR ;

BUCHANAN, JM .

JOURNAL OF CLINICAL INVESTIGATION, 1962, 41 (05) :1134-&

[6]

HOCHSTADTOZER J, 1971, J BIOL CHEM, V246, P5304

[7]

Huennekens F M, 1974, Adv Enzyme Regul, V12, P131, DOI 10.1016/0065-2571(74)90011-9

[8]

Huennekens F M, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P313

[9]

KESSEL D, 1978, J BIOL CHEM, V253, P400

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