CDNA CLONING OF THE HYDROXYSTEROID SULFOTRANSFERASE STA SHARING A STRONG HOMOLOGY IN AMINO-ACID-SEQUENCE WITH THE SENESCENCE MARKER PROTEIN SMP-2 IN RAT LIVERS

被引:104
作者
OGURA, K
KAJITA, J
NARIHATA, H
WATABE, T
OZAWA, S
NAGATA, K
YAMAZOE, Y
KATO, R
机构
[1] TOKYO COLL PHARM,DEPT HYG CHEM,DRUG METAB & TOXICOL LAB,HACHIOJI,TOKYO 19203,JAPAN
[2] KEIO UNIV,SCH MED,DEPT PHARMACOL,TOKYO 160,JAPAN
关键词
D O I
10.1016/0006-291X(90)91036-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA encoding hydroxysteroid sulfotransferase a (STa), which catalyzes activation of carcinogenic polycyclic hydroxymethyl-arenes, was isolated from a λgtll cDNA expression library constructed from poly(A)+RNA of a female Sprague-Dawley (SD) rat liver. The cDNA, designated as ST-40, consisted of 1,015 base pairs which had an open reading frame of 852 base pairs encoding the entire rat STa subunit of 284 amino acids. The nucleotide base sequence of the ST-40 cDNA shared a strong homology of 94.4% with that of ST-20 cDNA encoding a hydroxysteroid ST which had been reported by us. The deduced amino acid sequence of STa had a homology of 73.7% with that of an SD rat liver senescence marker protein (SMP-2) consisting of 282 amino acid residues. However, STa was found to share a much stronger homology of 92% on the average with SMP-2 in their four specific regions corresponding to about 60% of the total sequences, indicating SMP-2 to be an isozyme of hydroxysteroid ST. © 1990.
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页码:1494 / 1500
页数:7
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